1- Rory Gilmore (when she's in yale)
2- Wall-E
3- Robert Neville (but that isn't really the best example... He kind of dreams about having sex with vampires and drinks himself into stupors)
4- Jimmy Kudo (from the manga Case Closed. I mean... he has the professor man, but that's the only person that really knows it's him inside that kid body)
5- Horton the Elephant (He's was all alone when it came to protecting that lil clover... but then the he becomes friends with the entire jungle and the who world in the end)
6-Mark (from rent) He has all those great friends, but ends up isolating himself
7-Basically everyone from Spring Awakening (their whole society was messed up basically forcing them into isolation from each other and who they really were as people)
8-Chunky Rice and Dandel (from Good-bye, Chunky Rice) They have each other but then Chunky Rice has to move on. At least they miss each other together. That's kind of sweet.
9-Steve (Blue's Clues) I mean, really... all of his 'friends' are dogs or pieces of furniture. I'd say that qualifies him as alone.
10-Michael Jackson
11-Pocahantes (she has a whole tribe moves to a different world, only of her kind and then... dies)
12-Asterios Polyp
13-Leonard (Memento) I mean... he's not o.k. with being alone. He lost the love of his life. But none the less he goes on.
14-Spartacus (the movie. if you haven't seen it... watch it now)
15-Great White sharks
16-Rocks
17-Beta Fish (even though the males do make the bubble nests even when there is no girl around)
18-Giant Panda (they're UUBER aggressive and live in isolation)
19-Max Head Room
20-Tyler Durden/Narrator (Fight Club)
21- Judas Iscariot (I mean... he had all the disciples and Jesus and then betrayed them. Good going, Asshole)